Fluorescence-Based Assay for Demethylation
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- Categories
- Biotechnology, Diagnostics
- Summary
- WSU researchers have developed a fluorescence-based assay for the dealkylation activity of the non-heme iron (II) a-ketoglutarate-dependent dioxygenase family of enzymes. This assay uses formaldehyde dehydrogenase to convert formaldehyde to formic acid and monitors the creation of an NADH analog using fluorescence. The technology is a great improvement over the existing assays for DNA demethylation in that is continuous, rapid and does not require radioactively labeled material. Additionally, this assay may also be utilized to study the demethylation of histones.
This invention has been reported in T. W. Roy and A. S. Bhagwat, “Kinetic studies of E. coli AlkB using a new fluorescence-based assay for DNA demethylation,” Nucleic Acids Research, 2007, Vol. 35, No.21.
- File number
- 08-904
- Publications
- WSU is seeking a limited number of non-exclusive licensees of this research reagent technology.
- Contact
- Anne DiSante
Director, Technology Transfer Office
Phone: 313-577-5541
Fax : 313-577-2814
anne.disante@wayne.edu