Simple, Inexpensive Regulated Expression of Proteins in Yeast
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- Biotechnology
- Summary
- WSU faculty has developed a new method to regulate protein in expression in yeast, including a set of plasmids and yeast strains which can be used alongside other regulated expression systems. Expression systems that can be regulated are particularly useful because they allow an experimenter to control the timing and levels of gene expression. This approach can aid high throughput yeast two-hybrid assays, which rely on maintaining large libraries of expression strains, which are eventually mated to conduct assays for protein interactions. The technology is described in Finley, et.al, “Regulated expression of proteins in yeast using the MAL61-62 promoter and mating scheme to increase dynamic range,” Gene, Vol. 285 (1-2) (2002) pp. 49-57.
- File number
- 02-594
- Contact
- Anne DiSante
Director, Technology Transfer Office
Phone: 313-577-5541
Fax : 313-577-2814
anne.disante@wayne.edu