New Technology and Reagents to Study Protein Structure and Folding in Mammalian Cells
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- Categories
- Biotechnology
- Summary
- This invention is a new protein transduction method for efficient delivery of exogenous proteins into mammalian cells. In contrast to known protein transduction methods, this new approach has the capability of targeting transduced proteins to different cellular compartments, as well as protecting the proteins from degradation by cellular proteases. The reagent for delivery of the proteins is composed of cationic agents, lipids and enhancer in a carrier.
The Approach uses bacteria to produce recombinant proteins that are then modified with the reagent and delivered into mammalian cells. The mammalian cell machinery folds and post-translationally modifies the transduced proteins to produce native functional proteins that can be easily purified from the cells. The final high yield of properly folded and modified protein can be 35-60% of added bacterial expressed proteins.
This discovery provides a foundation for future applications of protein transduction technology, atomic resolution cell biology and protein drug therapy to treat human disease to name a few applications.
- File number
- 07-829, 07-830, 07-831
- Publications
- We are seeking industrial partners to further develop this technology. A patent application is on file.
- Contact
- Randy Ramharack
Technology Licensing Manager
Phone: 313-577-5541
Fax : 313-577-2814
randy.ramharack@wayne.edu